Waters UPLC Intact Mass Analysis Application Kit User Manual download pdf (Page 1)

CONTENTS

I. UPLC INTACT MASS ANALYSIS APPLICATION

KIT COMPONENTS

II. INSTALLING THE PRE-COLUMN TUBING INTO

MASSPREP MICRO DESALTING COLUMN

III. CONNECTING THE COLUMN TO LCT

PREMIER MS

IV. CONNECTING THE COLUMN TO Q-Tof

PREMIER MS, AND SYNAPT HDMS

V. CONNECTING THE COLUMN TO WATERS SQ

DETECTOR (SQD)

IV. SUGGESTED METHOD FOR MASS ANALYSIS

USING UPLC INTACT MASS ANALYSIS

APPLICATION KIT

a. Intact Antibody Analysis

b. Reduced Antibody Analysis

VII. TIPS FOR SUCCESS

UPLC Intact MASS Analysis Application Kit

(Part Number: 176001519)

I. UPLC INTACT MASS ANALYSIS APPLICATION

KIT COMPONENTS

Description Qty Part Number

MassPREP Micro Desalting Column 1 186004032

Desalting Cartridge Manual Assembly,

Cap/Tube/Column

1 430001988

UPLC Intact Mass Analysis Solution 1 715001664

Intact Mass Tubing Kit. Kit contains: 1 205000548

Peek Compression Screw, Long, 10–32 4 410002276

Peek Tubing, 0.062 OD x .004 ID x 8.50 L 1 430001991

Peek Tubing, 0.062 OD x .004 ID x 15.04 L 1 430001992

Peek Tubing, 0.062 OD x .004 ID x 22.20 L 1 430001993

[ CARE AND USE MANUAL ]

P/N: 430001988

P/N: 186004032

P/N: 410002276

P/N: 430001991

P/N: 430001992

P/N: 430001993

Figure 1. UPLC

Intact Mass Analysis Application Kit Components.

1 2 3 4 5 6 7 8

[ CARE AND USE MANUAL ] 1

Summary of Contents

Page 1 - [ CARE AND USE MANUAL ]

CONTENTS I. UPLC INTACT MASS ANALYSIS APPLICATION KIT COMPONENTS II. INSTALLING THE PRE-COLUMN TUBING INTO MASSPREP MICRO DESALTING COLUMNII

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II. INSTALLING THE PRE-COLUMN TUBING INTO MASSPREP MICRO DESALTING COLUMNRequired materials: 1/4-inch open-end wrench5/16-inch open-end wrenchF

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Figure 4. Fluidic configuration for LC-MS (UPLC-LCT Premier) analysis. A post-column salt diversion valve (top-left corner of the LCT Premier) can be

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Figure 5. Fluidic configuration for LC-MS analysis using mass spectrometers with no integrated salt divert valve.Figure 6. Column placement in the co

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2. Plug the terminal block into terminal block I/O (terminal block I/O is located on the back panel of the external divert valve).3. Connect the oth

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VI. SUGGESTED METHOD FOR MASS ANALYSIS USING UPLC INTACT MASS ANALYSIS APPLICATION KITMass Spectrometry has become a powerful tool for therapeu

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Figure 8. Total ion chromatograms (TICs) from Waters ACQUITY UPLC-LCT Premier ESI-TOF MS analyses of an intact IgG1, and pre and post blank runs. Col

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MS ConditionsThe MS parameters listed below can vary depending upon the physical characteristics of the analyzed proteins, flow rate, and MS probe pos

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Waters UPLC Intact Mass Analysis Application Kit User Manual Page 1

Browse online or download User Manual for Water equipment Waters UPLC Intact Mass Analysis Application Kit. Waters UPLC Intact Mass Analysis Application Kit User Manual

Summary of Contents

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Waters UPLC Intact Mass Analysis Application Kit User Manual Page 1

Browse online or download User Manual for Water equipment Waters UPLC Intact Mass Analysis Application Kit. Waters UPLC Intact Mass Analysis Application Kit User Manual

Summary of Contents

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