Waters Spherisorb Columns User Manual Page 1

[ Care and Use ManUal ]Waters Spherisorb Columns 1Waters spherisorb columnscontentsi. GettinG started a. Column Installation 1. Reversed-Phase Colu

[ Care and Use ManUal ]Waters Spherisorb Columns 101. Remove column.2. Use acetonitrile as mobile phase A, and acetonitrile with 0.05 mg/mL uracil

[ Care and Use ManUal ]Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.comWaters Spherisorb

[ Care and Use ManUal ]Waters Spherisorb Columns 2ChemistryParticle ShapeParticle Size (µm)Pore Size (Å)Surface Area (m2/g)Pore Volume (cc/g)% Carbo

[ Care and Use ManUal ]Waters Spherisorb Columns 3b. Column EquilibrationSpherisorb columns are shipped in test mobile phase. It is important to ensur

[ Care and Use ManUal ]Waters Spherisorb Columns 4ii. column useTo ensure the continued high performance of Spherisorb columns, follow these guideline

[ Care and Use ManUal ]Waters Spherisorb Columns 5Table 3: Buffer Recommendations for Using Spherisorb Columns from pH 2 to 8Additive or Buffer pKaBuf

[ Care and Use ManUal ]Waters Spherisorb Columns 6iv. troubleshootinGChanges in retention time, resolution, or backpressure are often due to column co

[ Care and Use ManUal ]Waters Spherisorb Columns 7buffer salt in the column when 100% acetonitrile is introduced. Note: The exception of the storage r

[ Care and Use ManUal ]Waters Spherisorb Columns 8Figure 3: Parker Ferrule in a Waters Style Endfitting Note: A void appears if tubing with a Parker s

[ Care and Use ManUal ]Waters Spherisorb Columns 9b. Band Spreading MinimizationFigure 6 shows the influence of tubing internal diameter on system ban