Waters Dye QCRM Kit User Manual download pdf

Dye Quality Control Reference Material Kit 1

[ CARE AND USE MANUAL ]

CONTENTS

I. INTRODUCTION

a. MS-Directed Purification

b. The Purpose of a QCRM

c. What Affects Your QCRM Results

II. STORAGE AND STABILITY

III. RECOMMENDED USAGE OF THE DYE QCRM

a. Testing System Performance

b. Performing the Delay Timing Test Manually

c. Performing a Chromatography Test

IV. TROUBLESHOOTING

V. ORDERING INFORMATION

DYE QUALITY CONTROL REFERENCE MATERIAL KIT

I. INTRODUCTION

The Quality Control Reference Materials (QCRM) portfolio is a unique

collection of standards and mixtures. These products allow users

to evaluate and benchmark the chromatography system before

analysis of critical material. The products in the portfolio are all

precisely formulated based on the expertise of Waters scientist.

The AutoPurification

™

Dye QCRM contains 3 compounds including

Thionin, Thioflavin T, and Crystal Violet.

This standard mix is used to confirm the benchmark performance

of a Preparative/Purification System through fraction collection.

This particular QCRM is precisely formulated to allow the user to

verify correct fraction collection. This test is highly valuable to

perform upon first use/installation, and any time the system

has not been used or has had any changes. These compounds were

vigorously tested and evaluated and chosen because they provide

the following advantages:

• Well-separated

• Easily visually identified

a. MS-Directed Purification

The Waters MS-directed autopurification system uses the Waters

SQD2 Mass Detector as its primary detector.

UV-Directed SystemMS-Directed System

PC with MassLynx and

FractionLynx software

2767 sample

manager

2545 binary

gradient module

Bottle

tray

2489 UV

detector

System fluidics

organizer

PC with MassLynx and

FractionLynx software

2767 sample

manager

2545 binary

gradient module

515 makeup pump

(not shown)

Bottle

tray

2998 PDA

detector

SQD2

detector

System fluidics

organizer

1 2 3 4 5 6 7 8

[ CARE AND USE MANUAL ] 1

Summary of Contents

Page 1 - [ CARE AND USE MANUAL ]

Dye Quality Control Reference Material Kit 1[ CARE AND USE MANUAL ]CONTENTSI. INTRODUCTION a. MS-Directed Purification b. The Purpose of a QCRM

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Dye Quality Control Reference Material Kit 2[ CARE AND USE MANUAL ]b. The Purpose of a Quality Control Reference MaterialWaters recommends to benchmar

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Dye Quality Control Reference Material Kit 3[ CARE AND USE MANUAL ]Note: When using a different size and type of column than the one described here, t

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Dye Quality Control Reference Material Kit 4[ CARE AND USE MANUAL ] Example: If the dye is seen in the waste line 15 seconds before the valve clicks

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Dye Quality Control Reference Material Kit 5[ CARE AND USE MANUAL ]• For a UV-directed system, enter 418 in the Wavelength A column and 590 in the Wa

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Dye Quality Control Reference Material Kit 6[ CARE AND USE MANUAL ]1. From the shortcut bar, click FractionLynx > Collection Control, and verify t

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Dye Quality Control Reference Material Kit 7[ CARE AND USE MANUAL ]V. ORDERING INFORMATIONTo order these products, contact your nearest subsidiary, or

Page 8

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Waters Dye QCRM Kit User Manual

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